重组大鼠 TNNI3 蛋白(His标记)
货号:106437 编号:XFBM490
CAS号: 规格:Tnni3; Troponin I; cardiac muscle; Cardiac troponin I
包装:10 μg 保质期:365天
保存条件:-20℃保存
DESCRIPTION | ||
Gene Name Synonym | Tnni3; Troponin I; cardiac muscle; Cardiac troponin I | |
Species | Rat | |
Accession | P23693 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | Ala2-Gly211 is expressed with a 10×His tag at the N terminus | |
Molecular Weight | 15.2 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
Tnni3 is the inhibitory subunit of the cardiac troponin (cTn) complex that is a sensor of the intracellular Ca2+ levels and modulates the interaction between the thick and thin filaments during muscle contraction. It mainly blocks the interaction between actin and myosin in the absence of Ca2+ and tightly regulated by phosphorylation. | ||
Data | ||
SDS-PAGE | Bioactivity | |
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Recombinant Rat TNNI3 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 11-15 kDa. | Immobilized Rat TNNI3, His Tag at 3 μg/mL (100 μL/well) can bind Anti-Cardiac Troponin I with a linear range of 0.009-0.078 μg/mL. | |
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