重组结核分枝杆菌 ESAT6-CFP10 融合蛋白 （His-tag）
货号：106443 编号：XFBM491
CAS号： 规格：esxA-esxB, esaT6, cfp10, lhp, mtsA10, ESAT6-CFP10
包装：10 μg 保质期：365天
保存条件：-20℃保存
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DESCRIPTION | ||
Gene Name Synonym | esxA-esxB, esaT6, cfp10, lhp, mtsA10, ESAT6-CFP10 | |
Species | Mycobacterium tuberculosis | |
Accession | P9WNK7 P9WNK5 | |
Source | E.coli | |
SPECIFICATIONS | ||
Amino acid sequence: | Met 1-Ala 95 and Met 1-Phe 100 fusion protein is expressed with a 6×His tag at the C terminus | |
Molecular Weight: | Approximately 23 kDa. | |
Purity: | >95.0% as determined by SDS-PAGE | |
Endotoxin: | < 1.0 EU/μg of the protein by the LAL method. | |
Formulation: | Lyophilized from sterile PBS, pH 7.4. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution: | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage： | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
Genes esxA and esxB are part of RD1 (part of a 15-gene locus known as ESX-1), a section of DNA deleted in the M.bovis BCG strain used for vaccination. Deletion of this region is thought to be largely responsible for the attenuation of BCG, and esxA and esxB in particular are very important in this effect.ESAT6 and CFP10 act as strong host (human) T-cell antigen. ESAT6is a secreted protein that plays a number of roles in modulating the host's immune response to infection as well as being responsible for bacterial escape into the host cytoplasm. ESAT6 Inhibits IL-12 p40 (IL12B) and TNF-alpha expression by infected host (mouse) macrophages, reduces the nitric oxide response by about 75%. Higher levels of recombinant protein inhibit IFN-gamma production by host (human) T-cells and also IL-17 and TNF-alpha production but not IL-2. CFP10 is a secreted protein that Involves in translocation of bacteria from the host (human) phagolysosome to the host cytoplasm. CFP10 might serve as a chaperone to prevent uncontrolled membrane lysis by its partner EsxA. ESAT6 and CFP10 May help regulate assembly and function of the type VII secretion system (T7SS). Individual ESAT6 and CFP10 proteins or ESAT6-CFP10 fusion protein can be used as strong antigenic proteins for tuberculosis diagnosis.
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Data | ||
SDS-PAGE | Bioactivity | |
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Recombinant Mycobacterium tuberculosis ESAT6-CFP10 fusion Protein was determined by SDS-PAGE with Coomassie Blue. | Fully biologically active determined by the dose dependent induction of TNF-alpha secretion in Raw264.7 cells. The ED50 is 0.1-0.5 μg/mL. | |
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