重组 SEB 蛋白（His-tag）
货号：106446 编号：XFBM492
CAS号： 规格：SEB, Enterotoxin type B, entB
包装：10 μg 保质期：365天
保存条件：-20℃保存
.png)
.png)
{{language.person.wx}}
.png)
{{language.person.phone}}
.png)
{{language.person.message}}
DESCRIPTION | ||
Synonym | SEB, Enterotoxin type B, entB | |
Species | Staphylococcus aureus | |
Accession | P01552 | |
Source | E.coli | |
SPECIFICATIONS | ||
Amino acid sequence | Glu28-Lys266 is expressed with a 6×His tag at the N,C terminus. | |
Molecular Weight | Approximately 30 kDa. | |
Purity | >95.0% as determined by SDS-PAGE. | |
Endotoxin | <1.0 EU/μg of the protein by the LAL method. | |
Formulation | Lyophilized from sterile Tris-Hcl, pH 8.0. Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
Staphylococcal Enterotoxin B (SEB) is an exotoxin produced by the bacterium Staphylococcus aureus. The superantigen Staphylococcal Enterotoxin B is a protein with a mass of 28.4 kDa. It is considerably more stable in non-physiological conditions than other protein-based toxins such as ricin and botulinum neurotoxins. As a bifunctional molecule, the superantigen SEB bond simultaneously with the receptor sites of Class II MHC molecules in the cell containing antigens and with the T-cell receptors (TCR) to activates the host immune system. In turn, this ternary complex activates a large number of T-lymphocytes initiating a systemic release of pro-inflammatory cytokines. Staphylococcal enterotoxins cause the intoxication staphylococcal food poisoning syndrome. The illness characterized by high fever, hypotension, diarrhea, shock, and in some cases death. | ||
Data | ||
SDS-PAGE | Bioactivity | |
|
| |
Recombinant SEB Protein was determined by SDS-PAGE with Coomassie Blue. | Measured by its ability to induce TNF-alpha secretion by THP-1 cells. The ED50 for this effect is 47 μg/mL. | |
快递服务
江浙沪皖用户邮费为10元，其他地区用户邮费为20， 国际运费请咨询sales@xfnano.com。购买满 500.0 元免运费。如果库存显示为0， 请电话或邮件和销售确认，免费热线电话：400 025 3200邮件：sale@xfnano.com 感谢您的支持！
推荐商品
