重组小鼠SIRP alpha(CD172A) 蛋白（His标记）
货号：106479 编号：XFBM473
CAS号： 规格：CD172a, BIT; MFR, MYD1, MYD-1, P84, PTPNS1, SHPS1
包装：10 μg 保质期：363天
保存条件：-20℃保存
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DESCRIPTION | |
Gene Name Synonym | CD172a, BIT; MFR, MYD1, MYD-1, P84, PTPNS1, SHPS1, SHPS-1, SHPS1CD172A, SIRPA, Sirp-alpha-1, SIRPalpha2, Sirp-alpha-2, Sirp-alpha-3 |
Species | Mouse |
Accession | P97797 |
Source | HEK293 cells |
SPECIFICATIONS | |
Amino acid sequence | Lys32-Asn373 is expressed with a 12×His tag at the C terminus |
Molecular Weight | 37.9 kDa（The protein migrates as 40-55 kDa under reducing condition (SDS-PAGE) due to glycosylation） |
Purity | >95.0% as determined by SDS-PAGE |
Endotoxin | <5 EU mg-1 |
Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. |
STORAGE | |
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
BACKGROUND | |
Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don′t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction. | |
Data | |
SDS-PAGE | Bioactivity |
| Testing in progress |
Recombinant Mouse SIRP alpha Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 45-90 kDa. | |
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