重组小鼠NCAM1(CD56) 蛋白（His标记）
货号：106491 编号：XFBM476
CAS号： 规格：CD56, MSK39, NCAM1, N-CAM-1
包装：10 μg 保质期：365天
保存条件：-20℃保存
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DESCRIPTION | ||
Gene Name Synonym | CD56, MSK39, NCAM1, N-CAM-1 | |
Species | Mouse | |
Accession | P13595 | |
Source | HEK293 cells | |
SPECIFICATIONS | ||
Amino acid sequence | Leu20-Thr711 is expressed with a 10×His tag at the N terminus | |
Molecular Weight | 78.5 kDa | |
Purity | >95.0% as determined by SDS-PAGE | |
Endotoxin | <5 EU·mg-1 | |
Formulation | Lyophilized from sterile PBS, pH 7.4.Normally 5%-8% trehalose and mannitol are added as protectants before lyophilization. | |
Reconstitution | Dissolved in sterile water, it is recommended to add 0.1% HSA or BSA for long-term preservation. | |
STORAGE | ||
Stability and Storage | 12 months from date of receipt at -20°C to -70°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for almost 6 months (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | |
BACKGROUND | ||
NCAM1 (Neural Cell Adhesion Molecule 1, also known as CD56) is a neural adhesion protein (NCAM) that belongs to the immunoglobulin superfamily.The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 is involved in neuron-neuron adhesion, neurite fasciculation, the outgrowth of neurites, etc. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.NCAM1 signaling is mediated either by homophilic or heterophilic interactions with fibroblast growth factor receptor (FGFR), L1-CAM, N-cadherin and other components of the extracellular matrix.Upon activation, NCAM1 triggers a variety of signaling cascades including FYN–focal adhesion kinase (FAK), MAPK, and phosphatidylinositol 3-kinase (PI3K) pathways. | ||
Data | ||
SDS-PAGE | Bioactivity | |
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Active Recombinant Mouse NCAM1 Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 80-140 kDa. | Immobilized Mouse NCAM1, His Tag at 10 μg/mL (100 μL/well) can bind Anti-NCAM1 with a linear range of 3.5-56.2 ng/mL. | |
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